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KMID : 0644120080210010083
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Journal of Oriental Gynecology
2008 Volume.21 No. 1 p.83 ~ p.98
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Inhibitory Effect of Soyosangagamhwajae on Melanin Synthesis and its Action Mechanism in B16F10 Mouse Melanoma Cell
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Kim Eun-Seop
Lim Hyun-Jung
Shin Sun-Mi
Kim Soo-Min
Lee Jung-Eun
Yoo Dong-Youl
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Abstract
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Purpose: This study was performed to determine the inhibitory effect of Soyosangagamhwajae(SYG) on melanin synthesis in B16F10 mouse melanoma cell. Methods: The Inhibitory effects of Soyosangagamhwajae(SYG) on melanin synthesis were determined by in-vitro assay. To elucidate inhibitory effects of SYG on melanin synthesis, we determined the melanin release in B16F10 cell. And to investigate the action mechanism, we assessed the gene expression of tyrosinase, TRP-1, TRP-2. PKA, in B16F10 cell. Results: 1. SYG significantly inhibited melanin-release in B16F10 cell. 2. SYG significantly inhibited mushroom tyrosinase activity in vitro. 3. SYG significantly suppressed the expression of tyrosinase in B16F10 cell. 4. SYG significantly suppressed the expression of TRP-1, TRP-2 in B16F10 cell. 5. SYG significantly suppressed the expression of PKA, in B16F10 cell. Conclusion: From these results, it may be concluded that SYG has the antimelanogenetic effect.
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KEYWORD
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Soyosangagamhwajae(SYG), antimelanogenetic effect, melanin synthesis, tyrosinase, TRP, PKA, B16F10 mouse elanoma cell.
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